Potato dextrose agar

Potato dextrose agar

Potato dextrose agar (FDA M127)(abbreviated "PDA") and potato dextrose broth (abbreviated "PDB") are common microbiological media made from potato infusion, and dextrose (corn sugar). Potato dextrose agar is the most widely used medium for growing fungi and bacteria which attack living plants or decay dead plant matter. [Harold Eddleman, Ph. D: Bacteria Media From Potato. Jan 1998. http://www.disknet.com/indiana_biolab/b029.htm. Accessed 29 May 2005.]

Potato infusion is made by boiling 200g of sliced (washed but unpeeled) potatoes in water for 30 minutes and then decanting or straining off the broth. Dextrose (corn sugar) is then added (20 g/L) and the medium is sterilized by autoclaving. [Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. [http://www.cfsan.fda.gov/~ebam/m127.html] .]

Common organisms that can be cultured on PDA are yeasts such as "Candida albicans" and "Saccharomyces cerevisiae" and molds such as "Aspergillus niger". [Merck KGaA: Potato Dextrose Broth. 2002. [http://www.emdchemicals.com/analytics/Micro_Manual/TEDISdata/prods/1_00510_0500.html#preparation] . Accessed 29 May 2005.]

ee also

* [http://www.cfsan.fda.gov/~ebam/bam-mi.html Bacteriological Analytical Manual Online]
* Atlas, R.M.: "Handbook of Microbiological Media", second edition. Lawrence C. Parks (1997)
*

References


Wikimedia Foundation. 2010.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • potato-dextrose agar — a culture medium containing potato infusion and glucose (dextrose), for culturing and inducing sporulation in molds …   Medical dictionary

  • Agar Papa Dextrosa —   Aspergillus niger …   Wikipedia Español

  • Agar plate — Infobox Laboratory equipment name = Agar plate caption = An agar plate streaked with microorganisms isolated from a deep water sponge. Individual colonies may be seen center right. acronym = other names = uses = Culturing inventor = manufacturer …   Wikipedia

  • agar — A complex polysaccharide (a sulfated galactan) derived from seaweed (various red algae); used as a solidifying agent in culture media; it has the valuable property of melting at 100°C, but not solidifying until 49°C. [Bengalese] bile salt a. an a …   Medical dictionary

  • MacConkey agar — with lactose (left) and non lactose (right) fermenters …   Wikipedia

  • Chocolate agar — showing Francisella tularensis colonies …   Wikipedia

  • Cysteine lactose electrolyte deficient agar — A clear CLED agar plate after cultivation …   Wikipedia

  • DCA agar — A microbiological culture of Salmonella sp. bacteria on DC agar culture medium DCA agar Deoxycholate Citrate Agar is a solid bacteriological growth medium. Uses It is particularly useful for the isolation of organisms that cause bacilliary… …   Wikipedia

  • Thayer-Martin agar — Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, note that the non selective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other… …   Wikipedia

  • Cetrimide agar — is a type of agar used for the selective isolation of the gram negative bacteria, Pseudomonas aeruginosa.[1] As the name suggests, it contains cetrimide, which is the selective agent against alternate microbial flora.[2] Cetrimide also enhances… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”